Use of Leuconostoc Mesenteroides to Produce a Dextran Bioflocculant

In this study, we aimed to determine the in vitro activity of Leuconostoc mesenteroides var. isolatedfrom sugar-industry effluents produce a dextran bioflocculant from sucrose as low-cost substrate.L. strains present residual cane juice sugar factory were isolated and biochemicallyidentified using Mayeux, Sandine, Elliker agar (MSE) selective medium. The strain number 3 (LM03) wasbiochemically identified L. mesenteroides, which was used for study. concentrationof quantified by dry weight, morphology purity evaluated Fourier-transforminfrared (FTIR) spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy(EDS). Flocculation via turbidimetric assays different pH ranges effluentsand doses dextran.To evaluate flocculant according effect pH, jar test kit Phipps Bird, USA, wasused with sample recollected effluent (sugar industry). samples adjusted 7, 8,9, 10 11, dose 40 ppm (dextran dose) at fast slow speed 150 50 rpm, respectively. Toevaluate influence dextran, values 5, 20 speeds 180–150rpm 30–50 respectively.The able highest concentration (26.87 g/L) 76 h incubation. Thepresence MSE after incubation characterized FTIR, SEM, EDS.Besides that, observed that best flocculation 9 40ppm agitation rpm 5 min 15 min,achieving 77.7% removal turbidity effluent.L. responsible bioflocculation